thiI [2019-08-08 13:41:18]

sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA

locus: BSU_29580

pI: 9.66

mw: 40.17 kDa

protein length: 401 aa

gene length: 1206 bp

function: biosynthesis of 4-thiouridine in tRNA

product: sulfuryl transferase

essential: no

synonyms: ytbJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 3,025,748 3,026,953

The protein

Catalyzed reaction/ biological activity

• receives a sulfuryl group from [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ] and transfers it to uridine at position 8 of tRNA [Pubmed|22773787]

Protein family

• thiI family (single member, according to UniProt)

Structure

• [PDB|4KR6] (ThiI-RNA complex from Thermotoga maritima) [pubmed|24705700]
• [PDB|2C5S] (from B. anthracis) [pubmed|16343540]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT
• BKK29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT

References

• 14567704,22773787,16343540,24705700